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rabbit anti ca v 1 2 polyclonal antibody  (Alomone Labs)
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A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
Rabbit Anti Ca V 1 2 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ca v 1 2 polyclonal antibody/product/Alomone Labs
Average 92 stars, based on 1 article reviews
rabbit anti ca v 1 2 polyclonal antibody - by Bioz Stars, 2026-02
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A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

Journal: PLoS ONE

Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

doi: 10.1371/journal.pone.0074719

Figure Lengend Snippet: A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

Article Snippet: Antibodies used were rabbit anti- Ca v 1.2 polyclonal antibody (Alomone, ACC-003-AG; 1:2000), mouse anti-NCX monoclonal antibody (SWANT, R3F1; 1:1000), mouse anti-SERCA2 monoclonal antibody (Affinity Bioreagents, MA3-919; 1:2000) and rabbit anti-Na/KATPase polyclonal antibody (Abcam; 1:2000).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

doi: 10.1371/journal.pone.0074719

Figure Lengend Snippet: Comparison of Key Ca 2+ Handling Mechanisms in Hearts and Cardiomyocytes From Young Adult OVX and Aged OVX Female Mice.

Article Snippet: Antibodies used were rabbit anti- Ca v 1.2 polyclonal antibody (Alomone, ACC-003-AG; 1:2000), mouse anti-NCX monoclonal antibody (SWANT, R3F1; 1:1000), mouse anti-SERCA2 monoclonal antibody (Affinity Bioreagents, MA3-919; 1:2000) and rabbit anti-Na/KATPase polyclonal antibody (Abcam; 1:2000).

Techniques: In Vivo, Activity Assay, Expressing